Fig. 2
From: The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis
Cell extracts were produced among 4 conditions. The appearance of whole cell lysate was presented for all phases of preparation (a). Flow cytometry analysis and propidium iodide (PI) staining of the extracts before and after 3 freeze–thaw cycles as well as after centrifugation. The patterns were represented as dot plot (b). Quantitative analysis of PI positive staining before (BF) and after (AF) freeze–thaw cycle was shown in bar chart (c). The concentration of protein in cell extracts were demonstrated comparing between conditions (d). All data were shown as mean ± SEM. (N = 3)