Fig. 5

Interaction between miRNA-6783-3p and circPRKD3 regulated osteogenesis of mechanically stimulated PDLSCs. (A) Validation of miR-6783-3p overexpression efficiency by qRT-PCR analysis in PDLSCs. (B-C) The mRNA expression level of ALP and RUNX2 in mechanically stimulated NC-mimics groups and mimics-miR-6783-3p groups after transfection for 24 h. (D-F) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in mechanically stimulated NC-mimics groups and mimics-miR-6783-3p groups after transfection for 24 h. Full-length blots were presented in Supplementary Fig. 4 of additional file 1. (G-I) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J after co-transfection of pLC5‐circPRKD3 plasmids and mimics-miR-6783-3p for 24 h under mechanical force. Full-length blots were presented in Supplementary Fig. 5 of additional file 1. (J) Predicted sites where circPRKD3 binds to different RUNX2 protein isoforms sequences on the catRAPID website. (a) Binding sites prediction on RUNX2 isoform a. (b) Binding sites prediction on RUNX2 isoform b. (c) Binding sites prediction on RUNX2 isoform d. (d) Binding sites prediction on RUNX2 isoform e. (K) The working model that miR-6783-3p acts as a sponge of circPRKD3 to indirectly regulate the osteogenesis of PDLSCs under mechanical force. Data are presented as mean ± SD of three independent experiments. (n.s., no significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)