Fig. 4

Screening and validation of circPRKD3 downstream targets. Immunofluorescence staining (A) and quantitative analysis (B) of circPRKD3 distribution in nucleus and cytoplasm. (C) Prediction of miRNAs combined with circPRKD3 through different algorithms. (D) Force-sensitive miRNAs were sought among the 10 predicted miRNAs above by qRT-PCR analysis. (E) CircPRKD3 overexpression-associated miRNAs among force-sensitive miRNAs were evaluated by qRT‐PCR in mechanically stimulated pLC5‐ciR-NC groups and pLC5‐circPRKD3 groups after transfection for 24 h. (F) CircPRKD3 knockdown-associated miRNAs among force-sensitive miRNAs were assayed by qRT‐PCR in mechanically stimulated sh-NC and sh-circPRKD3 groups after transfection for 24 h. (G) Schematic diagram of luciferase assay and related sequence information. (H) Relative luciferase activity was measured in circPRKD3-WT groups and circPRKD3-mut groups with or without miR-6783-3p mimics. Data are presented as mean ± SD of three independent experiments. (n.s., no significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)