Fig. 2

CircPRKD3 was upregulated during osteogenic development of mechanically stimulated PDLSCs. (A) Schematic diagram of mechanically stimulated PDLSCs in vitro. Morphology of PDLSCs without (B) or with (C) stretch inducement for 24 h (scale bar 200 μm). (D) Relative gene expression level of circPRKD3 in non-stretched and stretched-24 h groups. (E-F) The mRNA expression level of ALP and RUNX2 in non-stretched and stretched-24 h groups. (G-I) The protein level of ALP and RUNX2 and the quantitative data analyzed by image J in non-stretched and stretched-24 h groups. Full-length blots were presented in Supplementary Fig. 1 of additional file 1. (J) The genetic sequence of circPRKD3 on circBase. (K) Centroid plain structure drawing of circPRKD3. (L) Centroid structure drawing encoding positional entropy of circPRKD3. (M) MFE plain structure drawing of circPRKD3. (N) MFE structure drawing encoding positional entropy of circPRKD3. (O) Peak diagram of circPRKD3 with minimum free energy. Data are presented as mean ± SD of three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)