Fig. 5

SP1 knockdown ameliorated SCI and contributed to the polarization of LPS-induced BV2 microglia from M1 to M2 phenotype in vivo. SCI mouse model was established by hitting from a height of 3 cm and subdural injection of the corresponding lentivirus expressing sh-SP1 in the lesioned area was conducted 5 min after spinal hitting. (A) Basso Mouse Scale (BMS) score was used to functionally grade the mice in the Sham group, SCI group and SCI + sh-SP1 group. (B and C) Rotarod test was used to analyze motor function of each mice. (D and E) Western blotting assay was used to detect SP1 and HTR2B protein expression in the spinal cord tissues of each mouse. (F) HE staining assay was performed to analyze the pathological conditions of spinal cord tissues of each mouse. (G) Cell apoptosis of spinal cord tissues of each mouse was analyzed by TUNEL assay. (H) Western blotting assay was used to detect Bax and Bcl-2 protein expression. (I and J) qRT-PCR was performed to detect mRNA expression levels of TNF-α, IL-1β, IL-4 and TGF-β in the spinal cord tissues of each mouse. (K and L) iNOS, CD86, Arg-1 and CD206 protein levels were analyzed by western blotting assay in the spinal cord tissues of each mouse. *P < 0.05