Fig. 4

SP1 silencing promoted polarization of microglia M2 phenotype by decreasing HTR2B expression. (A) Western blotting assay was used to detect HTR2B protein expression in BV2 cells transfected with vector or oe-HTR2B. (B-I) BV2 cells were divided into LPS + sh-NC group, LPS + sh-SP1 group, LPS + sh-SP1 + vector group and LPS + sh-SP1 + oe-HTR2B group. (B) HTR2B protein expression was determined by western blotting assay. (C) MTT assay was used to analyze cell viability. (D) Tunel assay was performed to analyze cell apoptosis. (E) Western blotting assay was used to detect protein expression of Bax and Bcl-2. (F and G) qRT-PCR assays were conducted to analyze the mRNA expression levels of TNF-α, IL-1β, IL-4 and TGF-β. (H and I) iNOS, CD86, Arg-1 and CD206 protein levels were analyzed by western blotting assay. *P < 0.05