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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: SP1 transcriptionally activates HTR2B to aggravate traumatic spinal cord injury by shifting microglial M1/M2 polarization

Fig. 2

SP1 transcriptionally activated HTR2B in BV2 cells. (A) DNA binding motif of SP1 was obtained from the JASPAR online database. (B) The schematic illustration showed the binding sites of SP1 for HTR2B. (C) Western blotting assay was used to detect SP1 protein expression after transfection with vector or SP1 overexpression plasmid. (D and E) ChIP assay and dual-luciferase reporter assay were conducted to identify the association of SP1 and HTR2B. (F) The effect of LPS treatment on HTR2B protein expression was analyzed by western blotting assay. (G and H) Western blotting assay and qRT-PCR assay were performed to analyze the effects of abnormal SP1 expression on HTR2B expression. *P < 0.05

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X