Skip to main content
Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: SP1 transcriptionally activates HTR2B to aggravate traumatic spinal cord injury by shifting microglial M1/M2 polarization

Fig. 1

SP1 knockdown promoted the polarization of LPS-induced BV2 microglia from M1 to M2 phenotype in vitro. (A) The effect of LPS treatment on SP1 protein expression was analyzed by western blotting assay. (B) The effect of SP1 shRNA in downregulating SP1 protein expression was determined by western blotting assay. (C-J) BV2 cells were divided into control group, LPS group, LPS + sh-NC group, and LPS + sh-SP1 group. (C) SP1 protein expression was analyzed by western blotting assay. (D) MTT assay was used to analyze cell viability. (E) Tunel assay was performed to analyze cell apoptosis. (F) Western blotting assay was used to detect protein expression of Bax and Bcl-2. (G and H) qRT-PCR assays were conducted to analyze the mRNA expression levels of TNF-α, IL-1β, IL-4 and TGF-β. (I and J) iNOS, CD86, Arg-1 and CD206 protein levels were analyzed by western blotting assay. *P < 0.05

Back to article page

Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X