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Figure 7 | Journal of Orthopaedic Surgery and Research

Figure 7

From: Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells

Figure 7

Immunohistochemistry at 24 and 52 weeks. Safranin O staining (a, e), immunostaining for type II collagens (b, f), immunostaining for type X collagens (c, g), and IgG staining as controls (d, h) at 24 weeks (a–d) and 52 weeks (e–h). No positive Immunostaining for type X collagens was observed in the implanted area during the regeneration period. Scale bar =200 μm. Representative subjects of three experiments for each time course are shown.

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